χ²-BL300™ Bioluminescence system

Brain tissues have native fluorescence.  In the context of in vivo fluorescence measurements, the native fluorescence from the brain tissues is induced by the excitation and contributes to the background fluorescence.  Detecting bioluminescence signals has the advantage of detecting only the signals of the intended biosensors and thereby improves the signal-to-noise ratio (SNR).

The challenge is that, in general, the quantum efficiency of bioluminescent sensors is much lower than that of a fluorescent sensor and the signals are not abundant. Detection sensitivity is the key for acquiring generally weak bioluminescence signals.

χ²-BL300™ is designed to take advange of the SNR of bioluminescence and overcome the challenge in detection sensitivity.  χ²-BL300™ stands out in its single photon sensitivity. It has the capability of detecting bioluminescence resonance energy transfer (BRET) through an optical fiber with an implantable fiber tip in freely moving animals.